LOCUS       40924_37743        6645 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRS304 pRS-ADH1pro-5-1.2-vYFPdel, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Belmont BJ, Niles JC.
  TITLE     Inducible Control of Subcellular RNA Localization Using a Synthetic 
            Protein-RNA Aptamer Interaction
  JOURNAL   PLoS ONE 7 (10), E46868 (2012)
  PUBMED    23056498
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Belmont BJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2012) Biological Engineering, Massachusetts 
            Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, 
            USA
REFERENCE   3  (bases 1 to 6645)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6645)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "10"; pages: "E46868"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-SEP-2012) Biological Engineering, Massachusetts Institute of 
            Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        185..465
                     /label=TRP1 promoter
     CDS             466..1137
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1237..1692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1837..1853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1860..1878
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2173..2844)
                     /codon_start=1
                     /label=(3-F)Tyr-EGFP
                     /note="(3-F)Tyr-EGFP is a basic (constitutively
                     fluorescent) green fluorescent protein published in 2004, 
                     derived from Aequorea victoria."
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VT"
     promoter        complement(2934..4359)
                     /label=ADH1(long)
                     /note="Saccharomyces cerevisiae alcohol dehydrogenase
                     promoter"
     promoter        complement(4385..4403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4424..4440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4448..4464)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4472..4502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4517..4538)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4826..5414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5588..6445)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6446..6550)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaacgac attactatat atataatata ggaagcattt aatagacagc atcgtaatat
      241 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
      301 atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga
      361 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
      421 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
      481 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
      541 gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
      601 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
      661 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
      721 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
      781 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
      841 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
      901 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
      961 aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat
     1021 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
     1081 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
     1141 gttattactg agtagtattt atttaagtat tgtttgtgca cttgcctgcg gtgtgaaata
     1201 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
     1261 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1321 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1381 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1441 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1501 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1561 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1621 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1681 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1741 tgcgggcctc ttcgctatta cgccagctgg cgaagggggg atgtgctgca aggcgattaa
     1801 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     1861 aatacgactc actatagggc gaattggagc tcaagcttta acgaacgcag aattttcgag
     1921 ttattaaact taaaatacgc tgaacccgaa catagaaata tcgaatggga aaaaaaaact
     1981 gcataaaggc attaaaagag gagcgaattt ttttttaata aaaatcttaa taatcattaa
     2041 aagataaata atagtctata tatacgtata taaataaaaa atattcaaaa aataaaataa
     2101 actattattt tagcgtaaag gatggggaaa gagaaaagaa aaaaattgat ctatcgaagg
     2161 aattctagat taagtaacaa attctaacaa gaccatgtgg tctctctttt cgtttggatc
     2221 tttggataag gcagattgat aggataagta atggttgtct ggtaacaaga ctggaccatc
     2281 accaattgga gtattttgtt gataatggtc agctaattga acaccaccat cttcaatgtt
     2341 gtgtctaatt ttgaagttag ctttgatacc attcttttgt ttgtcagcag tgatgtaaac
     2401 attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccat cttctttaaa
     2461 atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact tgacttcagc
     2521 tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa cataaccttc
     2581 tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc tagcaaaaca
     2641 cattaaacca taacctaaag tagtgactaa ggttggccat ggaactggca atttaccagt
     2701 agtacaaatt aattttaagg tcaatttacc gtaagtagca tcaccttcac cttcaccgga
     2761 gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc
     2821 agtgaataat tcttcacctt tagacatatg ttctcgagtt ttgtgttgga tccaggccat
     2881 cacattccgt ccgtatcgac ctttctctgc ctggatccta gataattaga tcctgaaata
     2941 ttgtgcagaa aaagaaacaa ggaagaaagg gaacgagaac aatgacgagg aaacaaaaga
     3001 ttaataattg caggtctatt tatacttgat agcaagacag caaacttttt tttatttcaa
     3061 attcaagtaa ctggaaggaa ggccgtatac cgttgctcat tagagagtag tgtgcgtgaa
     3121 tgaaggaagg aaaaagtttc gtgtgcttcg agatacccct catcagctct ggaacaacga
     3181 catctgttgg tgctgtcttt gtcgttaatt ttttccttta gtgtcttcca tcattttttt
     3241 gtcattgcgg atatggtgag acaacaacgg gggagagaga aaagaaaaaa aaagaaaaga
     3301 agttgcatgc gcctattatt acttcaatag atggcaaatg gaaaaagggt agtgaaactt
     3361 cgatatgatg atggctatca agtctagggc tacagtatta gttcgttatg taccaccatc
     3421 aatgaggcag tgtaattggt gtagtcttgt ttagcccatt atgtcttgtc tggtatctgt
     3481 tctattgtat atctcccctc cgccacctac atgttaggga gaccaacgaa ggtattatag
     3541 gaatcccgat gtatgggttt ggttgccaga aaagaggaag tccatattgt acacccggaa
     3601 acaacaaaag gatatccgaa atattccacg gtttagaaaa aaatcggaaa agagcgcgga
     3661 ggggtgttac cccccttctc tactagcatt ggactttaat taatatatgt gcataggaga
     3721 agtgtaaagt tcccttccat attgtaacat aataaagtgc acacccaaat gaattgaaag
     3781 cgtactcaaa cagacaacca tttccagtgt tgtatgtacc tgtctattta tactggtagc
     3841 aaccctattg ctgtttcctc ttcaaagtac tctagcggtt atgcgcgtct caccttcaag
     3901 gtcatggtcg ctctattgtt cgcaccaccg gcaaactcgc gtctcgcaag tcttggctca
     3961 ttcttctagt atactcatgt tgcaaatgca ctcaggttct ttcggcaact taaataatga
     4021 caccagttgt cgtggtcgtc atcatcgcaa ccccaaccgg cattcttatt gcttctccaa
     4081 tctcgcccct tagcgcaggg taaaccttgg aaaatgcagg cgcaaaaaac tccgccgggc
     4141 acagcctcac gcccagcgtt atcgccgggc cggcaagagc gcgggtccgc cacagagtca
     4201 gcatgattgt gcaattgcgt aaactcgttt tttcggcgcc gcaaagccaa atacatcata
     4261 tcaacacttt tcactttatt tttcgttcga cccttatatt tgtcttttgc cttcatgctc
     4321 cttgatttcc tatttcattt accatcattt cttcgatcgc ggccgcggta cccagctttt
     4381 gttcccttta gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg
     4441 tgtgaaattg ttatccgctc acaattccac acaacatagg agccggaagc ataaagtgta
     4501 aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg
     4561 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4621 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg cccccctgac gagcatcaca
     4861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4921 tcccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     5041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5641 ccatagttgc ctgactgccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa
     6001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6601 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
